mbt2 cells

    BioMed Research International - Hindawi Publishing Corporation

    Figure 4 shows that MBT2 cancer cells could be killed with a 300 ms shot of approximately 10 W/cm 2, while the nontargeted cells required 35 W/cm 2 to achieve the same effect (>0.3 cm in diameter). Our results suggest that anti-Mucin 7/Au nanospheres are able to kill cancer cells with minimum laser energy.

    Interleukin-21 Gene Transfection Into Mouse Bladder Cancer .

    MBT2/IL-21 proliferated only when CD8+ T cells were depleted, whereas MBT2/IL-21 proliferation was totally abrogated in mice depleted of CD4+ T cells, natural killer cells or interferon-γ. Subcutaneous injection of MBT2/IL-21 treated with mitomycin C remarkably inhibited parental MBT2 tumor growth at the contralateral site.

    Interleukin-21 Gene Transfection Into Mouse Bladder Cancer .

    Purpose: We developed the genetically modified mouse bladder carcinoma MBT2 (American Type Culture Collection, Manassas, ia), which secretes interleukin-21, to investigate the functional activities of interleukin-21 in tumor immunity.Materials and Methods: The IL-21 gene was cloned from activated T cells by reverse transcriptase-polymerase chain reaction, inserted into an expression .

    Antitumor effect of simultaneous transfer of interleukin .

    To investigate the mechanism of antitumor effect in IL-12 and/or IL-18 secreting MBT2 cells, nude mice were challenged with MBT2/IL-12, MBT2/IL-18 and MBT2/Both . Among them, only MBT2/IL-12 could grow; thus, T cells seem to play an important role in the rejection mechanism of MBT2/IL-12.

    Molecular Imaging of Cancer Cells Using Plasmon-Resonant .

    tumor cells.[31] The MBT2 cell line is known to highly express Her2 molecules. As a control for the gene-expression-specific molecular imaging, we have transfected expression vector car-rying RNAi against Her2/neu into the MBT2 cells and screened for the stable clone as the control group cancer cells.

    -Detailed Information [IFO50041]-

    Link; Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4660

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    Evasion of immunosurveillance by genomic alterations of .

    Jul 24, 2017· Parental and derivative mouse bladder cancer cell line MBT2 cells were maintained in ATCC-formulated Eagle's Minimum Essential medium containing 10% FBS.

    Inhibition of Matrix Metalloproteinase-2 Expression and .

    MBT2 bladder carcinoma cells was obtained after the i.v. inoculation of halofuginone-treated cells as compared with the high metastatic activity exhibited by control untreated cells. Under the same conditions, there was almost no effect of halofuginone on the rate of MBT2 cell proliferation.

    Expression of chemokine/cytokine genes and immune cell .

    Immune cell recruitment to the iliac lymph nodes and bladder. The lack of induction of chemokine/cytokine genes by LGG did raise the question of whether LGG was able to attract immune cells into the bladder. Therefore immune cells in the bladder and draining lymph nodes were identified by …

    A model of orthotopic murine bladder (MBT-2) tumor .

    A model of orthotopic murine bladder (MBT-2) tumor implants. A single-cell suspension of 1.0 × 10 5 MBT-2 cells/0.02 ml was successfully injected into the submucosal layer of the bladder, with an incidence of after four experimental weeks. Inoculated tumor grew expansively into the bladder cavity from the bladder submucosa and invaded.

    Mbt 2 Cells | ATCC | Bioz

    Figure Legend Snippet: Attenuation of pulmonary metastasis of mouse bladder cancer cells by Aggrus knockdown. ( a ) Whole-blood samples (200 µl) in the reaction tubes were stirred at 1,000 rpm at 37°C and preincubated for 2 min, followed by MBT-2 cells addition (1.6 × 10 5 cells).

    RETROVIRUS TYPE C IN THE MOUSE BLADDER CARCINOMA CELL LINE .

    This MBT-2 tumor cell line is nowadays globally distributed. The cells have been and are still used to study various aspects of bladder cancer. While studying the phagocytic capacity of MBT-2 cells for BCG organisms by electron microscopic methods, the presence of this retrovirus was noticed.

    Inhibition of Matrix Metalloproteinase-2 Expression and .

    the MBT2 murine bladder carcinoma cell line (26)—MBT2-t50 (27, 28)—we demonstrated a strong inhibitory effect of halofuginone on the MMP-2 promoter activity. This inhibition resulted in a marked decrease of both ECM invasion, using the Matrigel invasion assay, and lung colonization by the MBT2-t50 bladder carcinoma cells.

    Targeting Poxvirus Decapping Enzymes and mRNA Decay to .

    (A and B) MBT2 bladder carcinoma cells (A) or 4T1 breast carcinoma cells (B) seeded in 12-well dishes (approximately 5 × 10 5 cells/well) were infected (300 pfu/well) with either WT VACV, D9-deficient VACV (ΔD9), or D10-deficient VACV (ΔD10). After 48 hr, cultures were lysed by freeze thawing and the amount of infectious virus quantified by .

    [Gene therapy by in vivo interferon-gamma gene transfer to .

    The effect of in vivo direct IFN-gamma gene transfer by a retrovirus vector to MBT2 tumors was also evaluated. After the subcutaneous inoculation of MBT2 cells into mice, a virus culture supernatant containing IFN-gamma gene was injected into the same tumor site once a day for 3 days.

    Increase of androgen-induced cell death and androgen .

    growth that results in biphasic growth curve of LNCaP cells (25). In addition, androgen can inhibit the growth of LNCaP-derived cells, which grow under androgen ablation condition for over 40

    Syngeneic Cell Line Reference Database | Taconic Biosciences

    Syngeneic Cell Line Reference Database Taconic Biosciences' inbred mice and GEMs on defined genetic backgrounds are widely used for syngeneic tumor studies, in which mouse tumor cells are engrafted into hosts from the same genetic background. Use the database of published references below to help you identify tumor cell lines and associated background strains in selecting the most …

    Inhibition of Bladder Carcinoma Angiogenesis, Stromal .

    Aug 15, 1999· Inoculation of MBT2-t50 Cells. MBT2-t50 cells were dissociated into a single cell suspension (10 6 cells/ml), and 2 × 10 5 cells were injected via a 25-gauge needle into the s.c. space on the dorsa of C3H/He male mice (Harlan Laboratories, Jerusalem, Israel), 6 weeks of age. The mice were divided into four groups receiving different amounts of .

    Inhibition of Tumor Growth in Association with .

    MBT2 cells and usually reached an average 0.5-cm diameter within 2 to 3 weeks of inoculation. During the 2 weeks when experimental groups received indomethacin and/or poly(l:C), tumor volume in control animals treated with PBS increased by approximately 50% (Chart 1). Histological assessment of the

    Molecular Imaging of Cancer Cells Using Plasmon-Resonant .

    The MBT2 cell line is known to highly express Her2 molecules. As a control for the gene-expression-specific molecular imaging, we have transfected expression vector car-rying RNAi against Her2/neu into the MBT2 cells and screened for the stable clone as the control group cancer cells.

    In vivo growth of transitional and renal cell carcinoma .

    MBT2 cells infected with various MOI of AdLacZ (MOI 0-200) were stained with 5-bromo-4-chloro-3-indolyl-ß-galactopyranoside (X-gal) and the number of blue-stained cells was counted. Based on the results of the gene transfection efficiency assay, MBT-2 cells were infected with AdVEGF-

    "Exploring Biomarkers for Point of Care Bladder Cancer .

    Two cell lines were used to determine if there was a statistical difference in the DNA detection threshold. A detection limit of between 600 cells/200μL and 300 cells/200μL was seen in both MB49 and MBT2 cells. Lastly, an in vivo study was done in which a group of mice was implanted with 75,000 MB49 cells.

    mbt2 cells,

    Through the action of two virus-encoded decapping enzymes .

    Through the action of two virus-encoded decapping enzymes (D9 and D10) that remove protective caps from mRNA 5-termini, Vaccinia virus (VACV) accelerates mRNA decay and limits activation of host defenses. right into a effective anti-tumor therapy. versions.

    mbt2 cells,

    T helper cell - Wikipedia

    The T helper cells (T h cells), also known as CD4 + cells, are a type of T cell that play an important role in the immune system, particularly in the adaptive immune system. They help the activity of other immune cells by releasing T cell cytokines. These cells help suppress or regulate immune responses.

    In Vitro and in Vivo Studies of FePt Nanoparticles for .

    Anti-Her2 antibody conjugated FePt nanoparticles demonstrated molecular expression dependent CT/MRI dual imaging contrast effect in MBT2 cell line and its Her2/neu gene knock out counterpart. Selective contrast enhancement of Her2/neu overexpression cancer lesions in both CT and MRI was found in tumor bearing animal after tail vein injection of .

    Mouse Bladder Carcinoma Cell Line - How is Mouse Bladder .

    MBT2 - Mouse Bladder Carcinoma Cell Line. Looking for abbreviations of MBT2? It is Mouse Bladder Carcinoma Cell Line. Mouse Bladder Carcinoma Cell Line listed as MBT2. Mouse Bladder Carcinoma Cell Line - How is Mouse Bladder Carcinoma Cell Line abbreviated?

    Tumor Cell Cytotoxicity of a Novel Metal Chelator

    Cytotoxicity assays. MBT2 and T24 are mouse and human bladder cancer cell lines, respectively. MBT2 cells were a generous gift from Dr N. Lattime (Thomas Jefferson University, Philadelphia, PA), and T24 cells were obtained from the American Type Culture Collection. MRC-5 cells were a gift from H. Blau (Stanford University, Stanford, CA).

    -Detailed Information [IFO50041]-

    Cell No. IFO50041: Cell Name : LOT No. 05132009: Lot Specification : distribution: Medium : Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO . # 10099) Temperature : 37 C: Cell Density at Seeding : 5 - 7 x 10^4 cells/ml: Methods for Passages : Cells were harvested after treatment wih 0.25% trypsin and 0.02% EDTA. Doubling Time : approx. 20 hrs.

    Exploring Biomarkers for Point of Care Bladder Cancer .

    more effective at creating a hypotonic solution for the cells. Two cell lines were used to determine if there was a statistical difference in the DNA detection threshold. A detection limit of between 600 cells/200µL and 300 cells/200µL was seen in both MB49 and MBT2 cells. Lastly, an in vivo study was done in which a group of mice was .

    CT26.WT ATCC ® CRL-2638™ Mus musculus colon carcinoma

    Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to .

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